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HiCEP Technology Benefits

HiCEP technology differs fundamentally from traditional hybridization methodologies. The benefits engendered by this approach in analyzing transcription product expression levels are described below (Tab. 1). Because HiCEP technology results in cDNA fragments originating from transcription products for which differences in expression have been detected among samples, it is suited for applications as diverse as searching for pharmacogenomic target genes, screening for pharmacogenomic candidate compounds, cloning full length cDNA strands highly useful for development of diagnostic DNA arrays, fabricating functional DNA arrays, or creating transgenic and knockout mice.


Table 1    Comparison with other gene expression analysis techniques
HiCEP Technology Hybridization
(Bead array)
Hybridization
(Chip array)
Target organisms All eukaryotes All eukaryotes Decoded organisms
Target genes All transcription products
Unknown genes OK
All transcription products
Unknown genes OK
Blob number limited
Known genes only
Information about genes Sequence, cDNA cloning library not required Sequence, cDNA cloning library not required Sequence, cDNA cloning library required
Detection sensitivity 1.5X difference
detection limit
2.0X difference
detection limit
2.0X difference
detection limit
Reproducibility High
Good results even with rare mRNA
Low
Difficult with rare mRNA
Low
Difficult with rare mRNA
Gene identification Cloning and decoding required.
Predictable after database creation.
Cloning and decoding required. Cloning and decoding not required.
Time required App.1 month App. 4 months App. 2 weeks

Expression of unknown genes

Methods such as EST analysis, SAGE, and DNA arrays are all used for studying transcriptomes, but these all yield only limited information about the genes in question. Hybridization is also a possibility, but sensitivity is limited. HiCEP  technology fills this gap by allowing comparison and detection of genes with differing levels of transcription product expressed between multiple samples regardless of whether or not sequence information is known. When necessary, we can decode the sequence for the target gene.

Excellent genetic analysis capabilities

HiCEP technology does not depend on knowledge accumulated about any given gene. It allow amplification and fragment peak detection without altering the obtained gene population. Each peak corresponds to one gene, so changes in expression level may be identified.

Exhaustive

It is thought that over 20,000 genes are expressed in each cell, although the quantity depends on the cell type. HiCEP technology can detect over 15,000 of these genes, or 80-85%, enabling the construction of broad expression networks.

Rare genes also detectable

Fragment analysis lies at the foundation of HiCEP technology. This both permits high detection sensitivity (differentials as low as 1.5X) and makes the technology highly effective at analysis of rarely expressed genes because it uses peak height to detect expression level changes.

Low cost

Expression profiles can be compared among experiments during systematic studies of single cells or organisms (stimulus response, longitudinal experiments, etc.). Since the technology does not require control samples, researchers may reduce the number of experiments without effecting data quality, thereby controlling cost.



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